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KMID : 1094719990040030195
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 3 p.195 ~ p.198
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Fed-batch culture ofAeromonas hydrophila for the production of poly(3-hydroxubutyrate-co-3-hydroxyhexanoate) using two carbon sources
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Lee Young
Lee Seung-Hwan
Lee Sang-Yup
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Abstract
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To produce polyhydroxyalkanoate (PHA) copolymer which consists of 3-hydroxy-butyrate (3HB) and 3-hydroxyhexanoate (3HHx) by cultivation ofAeromonas hydrophila, fed-batch cultures were done under several nutrient limiting conditions. With the results from flask cultures, fed-batch cultures were carried out to produce large amounts of PHA. In the fed-batch culture, firstly glucose was fed to grow cell, and then, oleic acid fed to stimulate PHA in the cell. The final cell concentration, PHA content, PHA concentration, and 3-hydroxy-hexanoate fraction in 38 hr were 48.9 g/L, 15.05 wt%, 7.36 g/L and 12.2 wt%, respectively, resulting in the productivity of 0.19 g/L-h under phosphate-limiting condition.
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KEYWORD
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polyhydroxyalkanoate, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), Aeromonas hydrophila, high cell density culture, biodegradable
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